Decreased hepatic phosphatidylinositol-3,4,5-triphosphate (PIP3) levels and impaired glucose homeostasis in type 1 and type 2 diabetic rats.
نویسندگان
چکیده
BACKGROUND/AIMS Phosphatidylinositol-3,4,5-triphosphate (PIP3) and phosphatidylinositol-4,5-biphosphate (PIP2) are two well-known lipid second messengers. Polyphosphoinositides have been implicated in the regulation of the signal transduction pathways involved in glucose metabolism using cell culture studies. However, there are no in vivo studies in the literature investigating the status of PIP3 and PIP2 in any of the tissues of diabetic animals. The liver plays an important role in the regulation of whole body glucose homeostasis. This study investigated whether hepatic PIP3 and/or PIP2 levels are altered in diabetes. METHODS Experiments were performed in streptozotocin-treated type 1 (T1D) and ZDF type 2 (T2D) diabetic rats. Blood glucose was determined utilizing glucose oxidase, glycosylated hemoglobin (GHb) using Glyco-Tek Affinity columns, and hepatic PIP3 and PIP2 concentrations by sandwich ELISA, and Akt phosphorylation and GLUT2 protein abundance by Western blotting. RESULTS Blood glucose and GHb were higher in T1D and T2D rats compared to controls. As compared to control animals, in livers from T1D and T2D rats PIP3 levels were reduced, AKT phosphorylation downregulated, and GLUT2 protein expression increased. PIP2 levels were unchanged. CONCLUSION PIP3 is decreased, AKT phosphorylation downregulated, GLUT2 protein expression increased and glucose homeostasis altered in livers of type 1 and type 2 diabetic rats.
منابع مشابه
Activation of Protein Kinase C- by Insulin and Phosphatidylinositol-3,4,5-(PO4)3 Is Defective in Muscle in Type 2 Diabetes and Impaired Glucose Tolerance Amelioration by Rosiglitazone and Exercise
Insulin resistance in type 2 diabetes is partly due to impaired glucose transport in skeletal muscle. Atypical protein kinase C (aPKC) and protein kinase B (PKB), operating downstream of phosphatidylinositol (PI) 3-kinase and its lipid product, PI-3,4,5-(PO4)3 (PIP3), apparently mediate insulin effects on glucose transport. We examined these signaling factors during hyperinsulinemic-euglycemic ...
متن کاملThe Effect of 6 Week Resistance Training Program on Foxo1 Expression in Liver Cells and Glucose and Insulin Levels in Type 2 Diabetic Rats
Background & objectives: Foxo1 is a transcription factor in insulin signaling in the liver. The function of Foxo1 is important in the ability of insulin to regulate hepatic glucose production and glucose homeostasis. The aim of this study was to determine the effect of resistance training program on Foxo1 expression in liver cells as well as glucose and insulin serum levels in male Wistar rat...
متن کاملOriganum Majoranum Extract Modulates Gene Expression, Hepatic and Renal Changes in a Rat Model of Type 2 Diabetes
The present study was conducted to test the effect of Origanum Majoranum Extract (OME) of leaves on alterations induced in a model of type 2 diabetic rats. Adult male Wistar rats were fed high fat diet for 3 weeks and injected a single dose of streptozotocin (35 mg/kg) intraperitoneally to induce type 2 diabetic rats. Diabetic rats were given aqueous extract of OME in a dose of 20 mg/kg orally ...
متن کاملOriganum Majoranum Extract Modulates Gene Expression, Hepatic and Renal Changes in a Rat Model of Type 2 Diabetes
The present study was conducted to test the effect of Origanum Majoranum Extract (OME) of leaves on alterations induced in a model of type 2 diabetic rats. Adult male Wistar rats were fed high fat diet for 3 weeks and injected a single dose of streptozotocin (35 mg/kg) intraperitoneally to induce type 2 diabetic rats. Diabetic rats were given aqueous extract of OME in a dose of 20 mg/kg orally ...
متن کاملThe Effect of Interval Training On G6Pase Expression in Hepatic Tissue, Glucose and Insulin of Obese Rats with Type 2 Diabetic
Background: The objective of this experimental study was to determine the effect of 6 weeks of high intensity interval training (HIIT) on G6Pase expression in liver tissue, serum insulin and glucose and insulin resistance in obese rats with type 2 diabetic and compared with non-diabetic obese group. Methods: 28 male Wistar rats aged 10 weeks (220 ± 10 g) were obesed by 6 weeks of high-fat diet...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology
دوره 30 6 شماره
صفحات -
تاریخ انتشار 2012